http://http://dspace.unitus.it:80 The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. 2013-05-20T01:28:02Z http://hdl.handle.net/2067/1415 Title: Comparative Proteomic Analysis of Hemocyanins in Dinocras cephalotes and Perla marginata (Plecoptera) Authors: Fochetti, Romolo; Amore, Valentina; Ubero-Pascal, Nicolás; Egidi, Maria Giulia; Timperio, Anna Maria Abstract: Hemocyanins are large oligomeric respiratory proteins found in many arthropods and mollusks. The overall expression of hemocyanin mRNA, revealed by studies on Plecoptera hemocyanin sequencing, has raised the question of whether the protein is expressed or not. In fact, the presence of expressed hemocyanin has only been reported in the literature for one species, Perla marginata (Panzer, 1799). In this paper, we report the presence of hemocyanin and hexamerin proteins in Dinocras cephalotes (Curtis, 1827), a species closely related to P. marginata. To assess the presence of hemocyanin, we used a reproducible and highly sensitive method based on liquid chromatography tandem mass spectrometry. We conclude that regardless of its putative function (respiratory, immune defense, storage protein), the hemocyanin is actually expressed in species in which its mRNA is present. Description: L'articolo è disponibile sul sito dell'editore http://www.entsoc.org 2010-12-31T23:00:00Z http://hdl.handle.net/2067/1842 Title: Proteomics and Transcriptomics Investigation on longissimus Muscles in Large White and Casertana Pig Breeds Authors: Murgiano, Leonardo; D'Alessandro, Angelo; Egidi, Maria Giulia; Crisa, Alessandra; Prosperini, Gianluca; Timperio, Anna Maria; Zolla, Lello Abstract: Consumer complaints against the blandness of modern lean meat and the frequent reference to the more strongly flavored meat that was available years ago have prompted reconsideration of high fatdepositing typical pig breeds. Casertana and Large White pig breeds are characterized by a different tendency toward fat accumulation as they exhibit opposite genetic and physiological traits with respect to the energy metabolism. These physiological differences were investigated in longissimus lumborum muscles through proteomics (2-DE, MS/MS) and microarray approaches. Data were analyzed for pathway and network analyses, as well as GO term enrichment of biological functions. As a result, Casertana showed a greater amount of proteins involved in glycolitic metabolism and mainly rely on fast-mobilizable energy sources. Large White overexpressed cell cycle and skeletal muscle growth related genes. Metabolic behavior and other implications are discussed. Description: L'articolo è disponibile sul sito dell'editore http://pubs.acs.org/ 2009-12-31T23:00:00Z