The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-18T20:21:20Z 2013-05-18T20:21:20Z Bongiorni, Silvia Chillemi, Giovanni Prosperini, Gianluca Bueno, Susana Signorelli, Federica Moioli, Bianca Pariset, Lorraine http://hdl.handle.net/2067/1893 2011-04-12T00:30:10Z 2011-04-07T22:00:00Z

Title: Transcriptomic analysis of two sheep breeds during lactation, using a new custom microarray platform Authors: Bongiorni, Silvia; Chillemi, Giovanni; Prosperini, Gianluca; Bueno, Susana; Signorelli, Federica; Moioli, Bianca; Pariset, Lorraine Abstract: We aim at understanding the genomic influence on milk quality and synthesis by comparing two sheep breeds using sheep-specific microarray technology. From sheep ESTs deposited at NCBI we generated a chip carrying about 22,000 non-redundant features in quadruplicate, achieving very good technical outcomes. Oligos were in situ generated on chip using the Combimatrix equipment. We analysed the mammary transcriptome in individuals of two sheep breeds at two lactation stages, to identify genes controlling milk production and metabolic pathways in which these genes are involved. With |FC|>1.4, and p-value≤0.05, 142 and 14 genes resulted differentially expressed in stages 01 and 02, respectively.

2011-04-07T22:00:00Z Crisà, Alessandra Marchitelli, Cinzia Pariset, Lorraine Contarini, Giovanna Signorelli, Federica Napolitano, Francesco Catillo, Gennaro Valentini, Alessio Moioli, Bianca http://hdl.handle.net/2067/1884 2011-04-08T00:30:37Z 2011-04-05T22:00:00Z

Title: Exploring polymorphisms and effects of candidate genes on milk fat quality in dairy sheep Authors: Crisà, Alessandra; Marchitelli, Cinzia; Pariset, Lorraine; Contarini, Giovanna; Signorelli, Federica; Napolitano, Francesco; Catillo, Gennaro; Valentini, Alessio; Moioli, Bianca Abstract: Aim of the present study was to investigate the genetic control of the fatty acid (FA) composition in milk from three breeds of sheep: Altamurana, Gentile di Puglia and Sarda. SNP within genes, encoding enzymes putatively involved in the synthesis and metabolism of milk fat, were selected for analysis, and the allele substitution effects were determined for 16 genes, that were polymorphic in the three sheep breeds, upon the milk fat composition. Four genes (alpha-1-antichymotrypsin-2; diacylglycerol O-acyltransferase homolog-2; propionyl Coenzyme A carboxylase, beta polypeptide; insulin-like growth factor-I) play a role in the desaturation of stearic FA into polyunsaturated fatty acids (PUFA). Furthermore, two genes (growth hormone receptor and zona pellucida glycoprotein-2) affect the variability of the total fat content, in addition to the butyric and stearic FA profile, and that the fatty acid synthetase gene has an influence on the medium-chain FA. Milk FA profiles play an important role in dairy sheep farming because they have a large effect on cheese characteristics and also because sheep milk may be marketed as a source of nutraceuticals as it contains higher levels of CLA than milk from other ruminants. The current study evaluated the global effects of a large number of SNP and haplotypes on traits that are not commonly investigated in sheep but that are potentially very useful for improving milk quality.

2011-04-05T22:00:00Z