Unitus DSpaceThe DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material.http://http://dspace.unitus.it:802013-05-24T19:45:20Z2013-05-24T19:45:20ZCompartmentalisation of T cells expressing CD8α and TCRβ in developing thymus of sea bass Dicentrarchus labrax (L.)Picchietti, SimonaGuerra, LauraSelleri, LuigiBuonocore, FrancescoAbelli, LuigiScapigliati, GiuseppeMazzini, MassimoFausto, Anna Mariahttp://hdl.handle.net/2067/16482011-06-28T10:25:57Z2007-12-31T23:00:00ZTitle: Compartmentalisation of T cells expressing CD8α and TCRβ in developing thymus of sea bass Dicentrarchus labrax (L.)
Authors: Picchietti, Simona; Guerra, Laura; Selleri, Luigi; Buonocore, Francesco; Abelli, Luigi; Scapigliati, Giuseppe; Mazzini, Massimo; Fausto, Anna Maria
Abstract: Eggs, larvae, post-larvae and sexually immature juveniles of the teleost Dicentrarchus
labrax (L.) were assayed for the expression of genes encoding the T cell receptor b and
CD8a. RT-PCR of RNA extracted from larvae revealed TCRb transcripts from day 25 posthatching
(ph) and CD8a transcripts from 26 days later.
At day 51 ph, CD8a and TCRb mRNAs were localised by in situ hybridisation in thymocytes
of the outer and lateral zones of the thymic paired glands. From day 75 ph onwards the
signal was mainly detected in the outer region, drawing a cortex–medulla demarcation.
In 1-year-old fish, CD8a+ and TCRb+ thymocytes almost filled the cortex and extended in
large cords in the medulla. A CD8a TCRb+ subcapsular lymphoid zone was evident near the
septa coming from the inner connective capsule that delimited the thymus.
The localisation of CD8a and TCRb transcripts demonstrated a compartmentalisation
of the juvenile thymus due to distinct localisation of thymocytes at different
developmental stages.
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