Unitus DSpace The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-21T01:40:46Z 2013-05-21T01:40:46Z Effect of mobilizing agents on mycoremediation and impact on the indigenous microbiota Giubilei, Maria Angela Leonardi, Vanessa Federici, Ermanno Covino, Stefano Sasek, Vaclav Novotny, Cenek Federici, Federico D'Annibale, Alessandro Petruccioli, Maurizio http://hdl.handle.net/2067/1408 2011-06-29T15:15:47Z 2008-12-31T23:00:00Z Title: Effect of mobilizing agents on mycoremediation and impact on the indigenous microbiota Authors: Giubilei, Maria Angela; Leonardi, Vanessa; Federici, Ermanno; Covino, Stefano; Sasek, Vaclav; Novotny, Cenek; Federici, Federico; D'Annibale, Alessandro; Petruccioli, Maurizio Abstract: BACKGROUND: Mobilizing agents (MAs) have been suggested to improve the fungal degradation of polycyclic aromatic hydrocarbons (PAHs) in soil. Three different MAs (Tween 20, Tween 80 and soybean oil) were investigated for their ability to stimulate contaminant degradation by either Phlebia sp. DABAC 9 or Allescheriella sp. DABAC1 in a soil spiked with amixture of PAHs. RESULTS: Phlebia sp. and Allescheriella sp. markedly differed in their growth capabilities under non-sterile conditions and withoutMAs (3.0 versus 0.1 μg ergosterol g−1 soil, respectively). However, soybean oil led to a 35-fold increase of Allescheriella sp. growth. Contaminant degradations by Phlebia sp. DABAC 9 and Allescheriella sp. DABAC 1 were best supported by soybean oil and Tween 20, respectively. Enumeration of cultivable bacteria and denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA showed that microbial density and biodiversity were positively affected by the mycoremediation especially with Allescheriella sp., the use of which led to an evident detoxification. CONCLUSIONS: Allescheriella sp. DABAC 1 appears to be a promising strain in the remediation of PAH-contaminated soils. The different response of the two fungi to MAs addition confirms the stringent need for a preliminary lab-scale assessment of fungus/MA combinations prior to application. Description: L'articolo è disponibile sul sito dell'editore: http://www.onlinelibrary.wiley.com 2008-12-31T23:00:00Z