The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-25T22:36:26Z 2013-05-25T22:36:26Z Gargani, Maria Pariset, Lorraine Soysal, M. Ihsan Ozkan, Emel Valentini, Alessio http://hdl.handle.net/2067/1895 2011-04-12T00:30:14Z 2011-04-07T22:00:00Z

Title: Genetic variation and relationships among Turkish water buffalo populations Authors: Gargani, Maria; Pariset, Lorraine; Soysal, M. Ihsan; Ozkan, Emel; Valentini, Alessio Abstract: The genetic variation and relationships among six Turkish water buffalo populations,typical of different regions was assessed using a set of twenty-six heterologous (bovine) microsatellite markers. Between 7 and 17 different alleles were identified per microsatellite in a total of 254 alleles. The average number of alleles across all loci in all the analyzed populations was found to be 12.57. The expected mean heterozygosity (HE) per population was between 0.5 and 0.58. Significant departures from Hardy-Weinberg equilibrium were observed for 44 locus-population combinations. Population differentiation was analyzed by estimation of the FST index (values ranging from 0.053 to 0.123) among populations. In the PCA analysis the Merzifon population showed the highest differentiation compared to the others. Also some individuals of the Danamandira population appeared clearly separated. Instead Afyon, Coskun, Pazar and Thural populations represented one single cluster. The assignment of individuals to their source populations, performed using the Bayesian clustering approach implemented in STRUCTURE 2.2 software, has evidenced a high differentiation of Merzifon and Danamandira populations as well. The results of this study could be useful for the development of conservation strategies of the Turkish buffalo.

2011-04-07T22:00:00Z Pariset, Lorraine Mariotti, Marco Nardone, Alessandro Soysal, M. Ihsan Ozkan, Emel Williams, J. L. Dunner, Susana Leveziel, Hubert Maróti-Agóts, Ákos Bodò, I. Valentini, Alessio http://hdl.handle.net/2067/1890 2011-05-30T05:42:46Z 2011-04-06T22:00:00Z

Title: Relationships of Podolic cattle breeds assessed by single nucleotide polymorphisms (SNPs) genotyping Authors: Pariset, Lorraine; Mariotti, Marco; Nardone, Alessandro; Soysal, M. Ihsan; Ozkan, Emel; Williams, J. L.; Dunner, Susana; Leveziel, Hubert; Maróti-Agóts, Ákos; Bodò, I.; Valentini, Alessio Abstract: Italian Maremmana, Turkish Grey and Hungarian Grey breeds belong to the same Podolic group of cattle, have a similar conformation and recently experienced a similar demographic reduction. The aim of this study was to assess the relationship among the analysed Podolic breeds and to verify whether their genetic state reflects their history. To do so, approximately 100 single nucleotide polymorphisms (SNPs) were genotyped on individuals belonging to these breeds and compared to genotypes of individuals of two Italian beef breeds, Marchigiana and Piemontese, which underwent different selection and migration histories. Population genetic parameters such as allelic frequencies and heterozygosity values were assessed, genetic distances calculated and assignment test performed to evaluate the possibility of recent admixture between the populations. The data show that the physical similarity among the Podolic breeds examined, and particularly between Hungarian Grey and Maremmana cattle that experienced admixture in the recent past, is mainly morphological. The assignment of individuals from genotype data was achieved using Bayesian inference, confirming that the set of chosen SNPs is able to distinguish among the breeds and that the breeds are genetically distinct. Individuals of Turkish Grey breed were clearly assigned to their breed of origin for all clustering alternatives, showing that this breed can be differentiated from the others on the basis of the allelic frequencies. Remarkably, in the Turkish Grey there were differences observed between the population of Enez district, where in situ conservation studies are practised, and that of Bandirma district of Balikesir, where ex situ conservation studies are practised out of the original raising area. In conclusion, this study demonstrates that molecular data could be used to reveal an unbiased view of past events and provide the basis for a rational exploitation of livestock, suggesting appropriate cross-breeding plans based on genetic distance or breeding strategies that include the population structure.

2011-04-06T22:00:00Z