The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-19T20:14:19Z 2013-05-19T20:14:19Z Pariset, Lorraine Mariotti, Marco Nardone, Alessandro Soysal, M. Ihsan Ozkan, Emel Williams, J. L. Dunner, Susana Leveziel, Hubert Maróti-Agóts, Ákos Bodò, I. Valentini, Alessio http://hdl.handle.net/2067/1890 2011-05-30T05:42:46Z 2011-04-06T22:00:00Z

Title: Relationships of Podolic cattle breeds assessed by single nucleotide polymorphisms (SNPs) genotyping Authors: Pariset, Lorraine; Mariotti, Marco; Nardone, Alessandro; Soysal, M. Ihsan; Ozkan, Emel; Williams, J. L.; Dunner, Susana; Leveziel, Hubert; Maróti-Agóts, Ákos; Bodò, I.; Valentini, Alessio Abstract: Italian Maremmana, Turkish Grey and Hungarian Grey breeds belong to the same Podolic group of cattle, have a similar conformation and recently experienced a similar demographic reduction. The aim of this study was to assess the relationship among the analysed Podolic breeds and to verify whether their genetic state reflects their history. To do so, approximately 100 single nucleotide polymorphisms (SNPs) were genotyped on individuals belonging to these breeds and compared to genotypes of individuals of two Italian beef breeds, Marchigiana and Piemontese, which underwent different selection and migration histories. Population genetic parameters such as allelic frequencies and heterozygosity values were assessed, genetic distances calculated and assignment test performed to evaluate the possibility of recent admixture between the populations. The data show that the physical similarity among the Podolic breeds examined, and particularly between Hungarian Grey and Maremmana cattle that experienced admixture in the recent past, is mainly morphological. The assignment of individuals from genotype data was achieved using Bayesian inference, confirming that the set of chosen SNPs is able to distinguish among the breeds and that the breeds are genetically distinct. Individuals of Turkish Grey breed were clearly assigned to their breed of origin for all clustering alternatives, showing that this breed can be differentiated from the others on the basis of the allelic frequencies. Remarkably, in the Turkish Grey there were differences observed between the population of Enez district, where in situ conservation studies are practised, and that of Bandirma district of Balikesir, where ex situ conservation studies are practised out of the original raising area. In conclusion, this study demonstrates that molecular data could be used to reveal an unbiased view of past events and provide the basis for a rational exploitation of livestock, suggesting appropriate cross-breeding plans based on genetic distance or breeding strategies that include the population structure.

2011-04-06T22:00:00Z Negrini, Riccardo Nicoloso, Letizia Grazia Maria Crepaldi, Paola Milanesi, Elisabetta Colli, L. Chegdani, F. Pariset, Lorraine Dunner, Susana Leveziel, Hubert Williams, J. L. Ajmone-Marsan, Paolo http://hdl.handle.net/2067/1910 2011-05-30T05:20:50Z 2011-04-12T22:00:00Z

Title: TRACEABILITY OF FOUR EUROPEAN PROTECTED GEOGRAPHIC INDICATION (PGI) BEEF PRODUCTS USING SINGLE NUCLEOTIDE POLYMORPHISMS (SNP) AND BAYESIAN STATISTICS Authors: Negrini, Riccardo; Nicoloso, Letizia Grazia Maria; Crepaldi, Paola; Milanesi, Elisabetta; Colli, L.; Chegdani, F.; Pariset, Lorraine; Dunner, Susana; Leveziel, Hubert; Williams, J. L.; Ajmone-Marsan, Paolo Abstract: The use of SNPs in combination with Bayesian statistics for the geographic traceability of cattle were evaluated using a dataset comprising 24 breeds from Italy,France,Spain, Denmark, the Netherlands,Switzerland and UK genotyped with 90 polymorphic markers. The percentage of correct assignment of the individuals to their Country of origin was 90%, with an average assignment probability of 93% and an average specificity of 92%. The higher value was observed for UK breeds (97% of correct assignment) while Swiss animals were the most difficult to allocate (77% of correct assignment). Tracing of Protected Geographic Indication (PGI) products, the approach correctly assigned 100% of Guaranteed Pure Highland Beef; 97% of “Vitellone dell’Appennino Centrale” breeds; 84% of Ternera de Navarra, and 80% of Boeuf de Chalosse. Methods to verify Products of Designated Origin (PDO) and Protected Geographic Indication (PGI) products will help to protect regional foods and promote the economic growth of marginal rural areas by encouraging the product on of high quality niche market foods.

2011-04-12T22:00:00Z