Unitus DSpace

Lymphocyte functions in dairy cows under hot environment

2011-01-25T10:06:28Z

Title: Lymphocyte functions in dairy cows under hot environment Authors: Lacetera, Nicola; Bernabucci, Umberto; Scalia, Daniela; Ronchi, Bruno; Kuzminsky, Giorgina; Nardone, Alessandro Abstract: This study was carried out to ascertain the effects of intense high environmental temperatures (HET) on lymphocyte functions in periparturient dairy cows. The study was undertaken from the beginning of March through the end of July 2003 in a commercial dairy unit located approximately 40 km north of Rome. Thirty-four Holstein cows were utilised in the study. Twenty-two of these cows gave birth in spring (SP cows), from 28 March to 30 April. The remaining 12 cows gave birth in summer (SU cows), between 15 June and 2 July. The two groups of cows were balanced for parity and were fed the same rations. Blood samples were taken 4, 3, 2 and 1 week before calving, and 1, 2 and 4 weeks after calving, in order to evaluate peripheral blood mononuclear cell (PBMC) function in vitro, and to determine plasma cortisol concentrations. After isolation, the PBMC were stimulated with mitogens and their response in terms of DNA synthesis and IgM secretion was measured. During spring, either the day (9– 20 h) or the night (21–8 h) temperature humidity index (THI) was below the upper critical THI (72) established for dairy cows. During summer, the mean daily THI values were 79.5±2.9 during the day and 70.1±4.7 during the night. Furthermore, during summer, three heat waves (a period of at least 3 consecutive days during which there were less than 10 recovery hours) occurred. Recovery hours were intended hours with a THI below 72. The first heat wave lasted 5 days, the second 6 days, and the third 15 days. Compared to the SP cows, over the entire periparturient period the extent of DNA synthesis and IgM secretion levels were lower (P ranging from <0.01 to 0.0001) and higher (P<0.01) respectively, in the SU cows. Before calving, the SU cows also presented higher (P<0.01)concentrations of plasma cortisol compared to the SP cows. This study indicates that the effects of HET on the immune response depend on the specific immune function under consideration, and that neuroendocrinal changes due to HET may play a role in the perturbation of immune functions

Fatty acids affect proliferation of peripheral blood mononuclear cells in dairy cows

2011-04-05T00:30:36Z

Title: Fatty acids affect proliferation of peripheral blood mononuclear cells in dairy cows Authors: Lacetera, Nicola; Kuzminsky, Giorgina; Morera, Patrizia; Basiricò, Loredana Abstract: In vitro studies were performed to assess the effects of bovine plasma fatty acids on proliferation of peripheral blood mononuclear cells (PBMC). PBMC from 6 Holstein heifers were cultured in media containing oleic (OA), palmitic (PA), stearic (SA), linoleic (LA), palmitoleic (POA), or linolenic (LNA) acid at concentrations mimicking different degree of lipomobilisation. Proliferation of PBMC was stimulated by concanavalin A or pokeweed mitogen. Concentrations of OA, PA, SA and LA mimicking moderate-intense lipomobilisation impaired PBMC proliferation. Concentrations of OA or LA mimicking low degree of lipomobilisation enhanced PBMC proliferation. None of the POA, and LNA concentrations affected proliferation of PBMC.

Fatty acids affect proliferation of peripheral blood mononuclear cells in dairy cows.

2011-04-05T00:30:29Z

Title: Fatty acids affect proliferation of peripheral blood mononuclear cells in dairy cows. Authors: Lacetera, Nicola; Kuzminsky, Giorgina; Morera, Patrizia; Basiricò, Loredana Abstract: In vitro studies were performed to assess the effects of bovine plasma fatty acids on proliferation of peripheral blood mononuclear cells (PBMC). PBMC from 6 Holstein heifers were cultured in media containing oleic(OA), palmitic (PA), stearic (SA), linoleic (LA), palmitoleic (POA), or linolenic (LNA) acid at concentrations mimicking different degree of lipomobilisation. Proliferation of PBMC was stimulated by concanavalin A or pokeweed mitogen. Concentrations of OA, PA, SA and LA mimicking moderate-intense lipomobilisation impaired PBMC proliferation. Concentrations of OA or LA mimicking low degree of lipomobilisation enhanced PBMC proliferation. None of the POA, and LNA concentrations affected proliferation of PBMC.