The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-19T14:44:43Z 2013-05-19T14:44:43Z Gutiérrez-Pesce, Patricia Bizzarri, Marco Rugini, Eddo De Pace, Ciro http://hdl.handle.net/2067/2200 2011-10-13T23:05:34Z 2010-12-31T23:00:00Z

Title: In vitro microtuberization for simulating the developmental physiology of underground storage organ in Helianthus tuberosus Authors: Gutiérrez-Pesce, Patricia; Bizzarri, Marco; Rugini, Eddo; De Pace, Ciro Abstract: The method described here allows microtuber induction on single nodal explants dissected from in-vitro grown plantlets from tubers of three Helianthus tuberosus clones: Violet de Rennes (VR), Ungheria-3B(CU-3B), and K8-HS142.

2010-12-31T23:00:00Z De Pace, Ciro Vaccino, Patrizia Caceres, Maria Eugenia Corbellini, Maria http://hdl.handle.net/2067/2197 2011-11-29T08:43:33Z 2010-12-31T23:00:00Z

Title: Development of valuable wheat inbred lines through the introduction of Dasypyrum villosum germplasm in their pedigree Authors: De Pace, Ciro; Vaccino, Patrizia; Caceres, Maria Eugenia; Corbellini, Maria Abstract: Selection of improved stable wheat inbred lines for low input production systems was achieved through the relevant combination of wheat and Dasypyrum villosum (Dv) germplasm using two approaches: (1) a re-evaluation of a tri-parental species breeding scheme, and (2) a modified bulk-breeding method. Both methods optimized selection outcome. The first breeding method was based on the synthesis of a T. turgidum var durum Dv hexaploid amphiploid, and crossing of that amphiploid to a bread wheat cultivar. The second breeding method was developed by bulk harvesting seeds from F1 and F2 from each biparental combination involving commercial wheat cultivars and wheat introgression breeding lines (IBLs) containing various amount of chromatin from Dv. In the first case, the M V amphiploid displayed typical adaptive traits of Dv such as high resistance to diseases and heading earliness, and the further crossing of the amphiploid to Triticum aestivum cv Chinese Spring (CS) provided wheat breeeding lines showing additional enhancements for grain size and grain end-use quality. GISH analyses revealed that Dv chromatin introgression occurred in some of them, coupled to chromosome-arm exchanges. Interestingly, three of the selected lines, after two years of genetic analyses and low-input field tests, showed dominance and genetic stability of the adaptive traits. In the second case, the F1s expressed the ‘useful’ Dv genes inherited from the IBLs, providing strong evidence that the Dv genes for trait enhancements are expressed not only in CS but also in ‘elite’ wheat genetic background. The F2 progenies displayed wide segregation offering ample opportunity for selecting the desired combination of the ‘elite’ traits with the traits expressed by the ‘useful’ Dv genes inherited through the IBLs.

2010-12-31T23:00:00Z De Pace, Ciro Pasquini, Marina Matere, Antonio http://hdl.handle.net/2067/1730 2011-11-29T09:28:10Z 2006-12-31T23:00:00Z

Title: Genetic information on crop varieties provide high quality indicators to address agri-environmental issues Authors: De Pace, Ciro; Pasquini, Marina; Matere, Antonio Abstract: Evidences are provided that driving force sustainability indicators can be standardized from genetic information on: (a) Triticum aestivum (bread wheat) response to powdery mildew disease caused by Blumeria (syn. Erysiphe) graminis f. sp. tritici , and (b)T. turgidum var. durum (durum wheat) field response to brown rust disease caused by Puccinia triticina.

2006-12-31T23:00:00Z Catarcione, Giulio Vittori, Doriano Ciaffi, Mario Rugini, Eddo De Pace, Ciro http://hdl.handle.net/2067/1727 2011-06-29T17:17:51Z 2008-12-31T23:00:00Z

Title: The ‘Evergrowing’ genotype of Corylus avellana is expressed in the offspring of ‘Tonda Gentile Romana’, ‘Nocchione’ and ‘Tonda di Giffoni’ Authors: Catarcione, Giulio; Vittori, Doriano; Ciaffi, Mario; Rugini, Eddo; De Pace, Ciro Abstract: The hazelnut (Corylus avellana L.) ‘evergrowing’ phenotype (EVG) fails both to cease growth and to enter dormancy under the dormancy-inducing (i.e., short days) conditions suitable for the wild type. The EVG phenotype is expressed by the homozygous genotype for the recessive allele EVG-d. Description: L'articolo é disponibile sul sito dell'editore: http://www.ishs.org

2008-12-31T23:00:00Z Rugini, Eddo De Pace, Ciro Gutierréz-Pesce, Patricia Muleo, Rosario http://hdl.handle.net/2067/2181 2011-08-03T12:58:16Z 2010-12-31T23:00:00Z

Title: Olea Authors: Rugini, Eddo; De Pace, Ciro; Gutierréz-Pesce, Patricia; Muleo, Rosario Abstract: The genus Olea contains about 30 species were grouped into three subgenera, Tetrapilus, Paniculatae, and Olea (cultivated olive and wild relatives), found in Asia, Australia and Asia, Africa and Europe, respectively. The species O. europaea L. includes six subspecies: Olea europaea L. ssp. europaea (the Mediterranean olives); O. e. laperrinei (distributed in Saharan massifs of Hoggar, Aïr, Jebel Marra in Algeria); O. e. cuspidata (which moved from South Africa to Egypt, East Australian areas and Hawaii, and from Arabia to northern India and Southwest China); O. e. guanchica (Canary Islands); O. e. maroccana (southwestern Morocco); and O. e. cerasiformis (Madeira). Using molecular markers, it has been ascertained that the Mediterranean olives include the cultivated types (O. europaea L. ssp. europaea var. sativa), the true wild oleaster (O. e. e. var. sylvestris), and the feral form olevaster from seedlings raised from seeds of the cultivated types. The oleaster has a narrow range of distribution and it is often mistaken for olevaster. Recolonization of the Mediterranean basin by Oleaster occurred after the last glacial event, from refuges located in both eastern and western Mediterranean basin areas toward southern Europe. Oleaster is a source of rootstock for propagating new improved cultivated varieties. Cultivated and wild forms have the same diploid chromosome number (2n = 46) and are fully interfertile. Triploid and tetraploid genotypes have been isolated from cultivated O.e.e., but polyploid forms have been found in endangered natural populations of O. e. guancica (tetraploid) and O. e. maroccana (hexaploid). Individual oleaster trees showing superior performance for size and/or oil content of fruit were selected empirically during olive domestication and propagated vegetatively as clones using cuttings that were planted directly or, more recently, grafted onto indigenous oleasters. Genetic markers linked for most important agronomic traits, such as size of the tree, content of secondary products of fruit, flowering induction, oil quality, and biotic and abiotic resistance, will help introgression by conventional breeding of oleaster trait-enhancing genes into cultivated olive. Successful results were difficult to achieve due to both the complex genetic basis of the traits to be improved and the long juvenile period of the progenies that delays the expression of the target traits. In vitro techniques to regenerate doubled haploids from hybrids or somaclonal variation induction may complement classical breeding procedures. Genetic transformation could speed up the development of new genotypes, and transgenic olive plants with modified growth habit and putative induced disease resistance are being tested under filed conditions. However, the development of an efficient regeneration method from mature tissue is the limiting factor for the routine application of this technology to olive genetic improvement. Description: L'articolo è disponibile sul sito dell'editore http://www.springerlink.com

2010-12-31T23:00:00Z Vaccino, Patrizia Banfi, Riccardo Corbellini, Maria De Pace, Ciro http://hdl.handle.net/2067/1762 2011-11-29T09:30:03Z 2009-12-31T23:00:00Z

Title: Improving the Wheat Genetic Diversity for End-Use Grain Quality by Chromatin Introgression from the Wheat Wild Relative Dasypyrum villosum Authors: Vaccino, Patrizia; Banfi, Riccardo; Corbellini, Maria; De Pace, Ciro Abstract: Dasypyrum villosum chromatin, including genes at the Glu-V1 locus, signify cantly improves wheat bread-making quality, and chromatin from the short arm of chromosome 6V also improves protein and micronutrient content. Description: L'articolo è disponibile sul sito dell'editore: http://www.crops.org

2009-12-31T23:00:00Z Caceres, Maria Eugenia Vaccino, Patrizia Corbellini, Maria Cionini, Pier Giorgio Sarri, Vania Polizzi, Enza Vittori, Doriano De Pace, Ciro http://hdl.handle.net/2067/1763 2011-06-29T17:23:09Z 2007-12-31T23:00:00Z

Title: Flowering earliness in wheat inbred breeding lines derived from T. aestivum ‘Chinese Spring’ x Dasypyrum villosum hybridization is not related to allelic variation at the vernalization loci VRN-A1, VRN-B1, and VRN-D1 Authors: Caceres, Maria Eugenia; Vaccino, Patrizia; Corbellini, Maria; Cionini, Pier Giorgio; Sarri, Vania; Polizzi, Enza; Vittori, Doriano; De Pace, Ciro Abstract: Substantial cryptic chromosome mutations or recombinations or gene mutations have occurred during the earlier generations following the hybridization T. aestivum “Chinese Spring” (CS) x Dasypyrum villosum event. Those genomic modifications were transmitted to the progeny plants, from which wheat introgression breeding lines (IBL) were derived. One of the mutations might have affected a flowering-promoting gene at a locus different from VRN-1, causing (under nonvernalizing condition and 13-14-hour daylength) the IBL “CSxV59” and the F1 hybrids “CSxV59 x cv Salgemma" and “CSxV59 x cv Isengrain” to start anthesis in less than 50 days from sowing compared to CS and to the two parental winter bread wheat cv Salgemma and cv Isengrain, which flowered about 150 days later.

2007-12-31T23:00:00Z Kuzmanović, Ljiljana Delfini, Maurizio Rugini, Eddo Gutiérrez-Pesce, Patricia De Pace, Ciro http://hdl.handle.net/2067/1768 2011-06-30T13:00:41Z 2008-12-31T23:00:00Z

Title: Micro-methods for genotypic screening of hazelnut (Corylus avellana L.) seeds to accelerate breeding for seed metabolite improvement Authors: Kuzmanović, Ljiljana; Delfini, Maurizio; Rugini, Eddo; Gutiérrez-Pesce, Patricia; De Pace, Ciro Abstract: We used hazelnut full-sib (FS) seed progenies from controlled pollination for efficiency testing of a multi-micro-method procedure comprising: a generalized low reagent-cost and low amount of extracting-tissue protocol for DNA purification and SSR seed genotyping, nuclear magnetic resonance (NMR) detection of analyte spectra for phenotyping metabolite content, and in vitro culture for clonal propagation of the embryo genotype.These micro-methods can be integrated into breeding programs for hazelnut fruit metabolite improvement. Description: L'articolo é disponibile sul sito dell'editore: http://www.ishs.org

2008-12-31T23:00:00Z Kuzmanović, Ljiljana Rugini, Eddo De Pace, Ciro http://hdl.handle.net/2067/1769 2011-06-28T11:46:04Z 2008-12-31T23:00:00Z

Title: Biometrical Methods to Reinforce Selection Efficiency in Breeding Programs for Hazelnut (Corylus avellana L.) Crop Improvement Authors: Kuzmanović, Ljiljana; Rugini, Eddo; De Pace, Ciro Abstract: The regression of half-sib (HS) offspring compared to the mother plant from accessions of ‘Tonda Gentile Romana’, ’Nocchione’, ‘Tonda Gentile delle Langhe’, ‘Nociara’, ‘San Giovanni’, ‘Longue d’Espagne’, ‘Sivri A’, and ‘Karidati’, was used as a method to estimate heritability for the following traits: nut weight, nut width, nut shape index, seed weight, husk length and nut length of hazelnut landraces. Nut length showed the highest and significant offspring-one parent regression coefficient. The progeny from ‘Longue d’Espagne’and ‘Tonda Gentile Romana’ are the most promising for selection progress. Description: L'articolo é disponibile sul sito dell'editore: http://www.ishs.org.

2008-12-31T23:00:00Z Kuzmanović, Ljiljana Rugini, Eddo De Pace, Ciro http://hdl.handle.net/2067/1765 2011-06-29T17:28:08Z 2008-12-31T23:00:00Z

Title: A generalized procedure to prepare PCR-grade DNA from different Corylus avellana L. plant tissues for parentage analysis. Authors: Kuzmanović, Ljiljana; Rugini, Eddo; De Pace, Ciro Abstract: A generalized protocol has been developed for an easy DNA purification from different hazelnut tissues. The extraction method avoids the use of liquid nitrogen, requires minimal amount (50 mg) of plant tissue, and allows the recovery of 0.8-1.3 μg high molecular weight and PCR-grade DNA from cotyledons and leaves and 0.2-0.3 μg of DNA from the pollen. The purified DNA has the quality to PCR-amplify and is neatly visualized in agarose gels. This protocol could improve and speed-up not only parentage analysis but also the application of marker assisted selection in hazelnut breeding programs, embryo genotyping before seed germination, analysis of the segregation pattern in the seed-progeny originating from the same mother plant, and detection of association among genetic molecular markers and phenotypic traits of cotyledons and embryo axis. Extension of the described procedure to molecular analysis of other difficult tree species is conceivable. Description: L'articolo è disponibile sul sito dell'editore: http://www.ishs.org

2008-12-31T23:00:00Z Kuzmanović, Ljiljana Rugini, Eddo De Pace, Ciro http://hdl.handle.net/2067/1770 2011-06-30T17:01:54Z 2008-12-31T23:00:00Z

Title: Allele segregation at SSR loci and allele traceability for hazelnut accessions (Corylus avellana L.) from landraces grown in the Latium Region (Italy) Authors: Kuzmanović, Ljiljana; Rugini, Eddo; De Pace, Ciro Abstract: Allele differences at six SSR loci among accessions of hazelnut landraces cultivated in the Latium region in Italy (‘Tonda Gentile Romana’, ‘Tonda di Giffoni’ and ‘Nocchione’) were determined using: (a) full-sib (FS) progenies from either controlled pairwise pollination or open pollinations of those accessions, (b) DNA purification and SSR-PCR analysis from seeds of each offspring, (c) segregation analysis of SSR-amplicons, and (d) allele annotation to the segregating amplicon database. The polymorphic PCR fragments amplified at each SSR locus favoured parentage analysis, DNA fingerprinting of the parental accessions, traceability of hazelnut seed products, detection of associations to economically important seed traits, and inference on genetic systems affecting pollen tube growth. Description: L'articolo è disponibile sul sito dell'editore: http://www.ishs.org

2008-12-31T23:00:00Z De Pace, Ciro Del Puglia, Salvatore Ruggeri, Roberto Rossini, Francesco Vittori, Doriano http://hdl.handle.net/2067/2196 2011-10-13T09:18:47Z 2009-12-31T23:00:00Z

Title: New clones of Helianthus tuberosus enlarge options for the sustainable integration of bioenergy crops in current farming systems of central Italy Authors: De Pace, Ciro; Del Puglia, Salvatore; Ruggeri, Roberto; Rossini, Francesco; Vittori, Doriano Abstract: Among the few species eligible to become dedicated bioenergy crops, Helianthus tuberosus L. (Ht) may become an alternative to dismissed maize or sugar beet or grape cropping systems. The efficiency of Ht stalk and tuber biomass production depends, among other factors, on plant density and water resources supply. Ht clones expressing new structure for plant architecture and phenological phases were selected to explore a range of farm production environments in Central Italy, differing mainly for the air temperature and rainfall patterns. In those environments the new clones are suitable for high stalk-biomass and high tuber-biomass production to be transformed in biofuel.

2009-12-31T23:00:00Z Acampora, Andrea Ciaffi, Mario De Pace, Ciro Paolacci, Anna Rita Tanzarella, Oronzo A. http://hdl.handle.net/2067/1616 2011-06-30T14:41:50Z 2006-12-31T23:00:00Z

Title: Pattern of variation for seed size traits and molecular markers in Italian germplasm of Phaseolus coccineus L. using different molecular markers. Authors: Acampora, Andrea; Ciaffi, Mario; De Pace, Ciro; Paolacci, Anna Rita; Tanzarella, Oronzo A. Abstract: Variation in Italian germplasm of Phaseolus coccineus L. was assessed for seed traits and molecular markers. A total of 130 seeds and seedlings, five for each of 21 Italian landraces, an Italian commercial cultivar and four Mesoamerican landraces of P. coccineus, were analysed using seven selected PCR markers: three RAPDs, two ISSRs and two ETs. Seed weight of the Mesoamerican landraces was £1 g, whereas that of the Italian landraces varied from 1 g to 2.5 g and was related to their origin. Oval shape was more frequent, with round shape observed only in Mesoamerican landraces. Three seed coat colours were observed: white, violet mottled or spotted black and buff spotted brown, also this trait was related to the origin. The level of polymorphism detected by molecular markers was low but with significant discriminant power. ISSRs were the most effective markers prone to unravel molecular polymorphism. The within accession component of variation exceeded that among accessions, as expected for an allogamous species. However correct classification of the individuals was achieved performing either discriminant analysis of the seed phenotypic traits or cluster analysis of seedling similarity measure based on the whole banding patterns obtained by the three marker types. Our data suggest that the Italian farmers, starting with ancestral Mesoamerican runner bean introductions in Europe, bred their own landraces through selection for seed size and seed coat colour, but occasional gene flow maintained variability within landraces bred by different farmers in the same Italian Region. Selection favored molecular and seed trait uniformity within several landraces making them suitable for certification. Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com

2006-12-31T23:00:00Z Corbellini, Maria Vaccino, Patrizia Banfi, Riccardo Coppa, Tania De Pace, Ciro http://hdl.handle.net/2067/2198 2011-11-29T09:33:39Z 2010-12-31T23:00:00Z

Title: Stabilità e capacità produttiva di linee inbred di frumento contenenti geni o cromosomi di D. villosum Authors: Corbellini, Maria; Vaccino, Patrizia; Banfi, Riccardo; Coppa, Tania; De Pace, Ciro Abstract: High yielding and good bread-making quality varieties are needed to improve common wheat cultivation in North and Central Italy. We evaluated advanced wheat intragression breeding lines (IBL), obtained from wheat Dasypyrum villosum (Dv)hybridization and backcross to the wheat parent, in order to achieve introgression of small portions of the Dv genome into the wheat nuclear genome. The IBLs were included in three sets of field trials (A, B, and C), each arranged according to a randomized block design, in three locations. The agronomic performance of the IL over the tested environments evidenced three main results: A) the IL 41-03, 8-1, and Mut 3-04, are ready to enter the registration process for releasing new high yielding and good quality wheat varieties; B) the IL CS V58, CS V59, CS V60 showed early heading, and the IBL CS V32 and CS V63 showed resistance to foliar diseases; the grain yield and phenotypic stability of all of them were as good or superior to the wheat parent “Chinese Spring”, which suggest that they will be useful donors of genes for heading earliness (CS V60 was at least 8 days earlier than both “Chinese Spring” and the earliest check) and disease resistance in future wheat breeding programs; C) An IBL derived from the T. turgidum var durum cv “Modoc” Dv (M V)amphiplod is early heading, with tenacious glumes, and high protein content suitable as a new “farro” for low – input farming systems.

2010-12-31T23:00:00Z Vaccino, Patrizia Coppa, Tania Corbellini, Maria De Pace, Ciro http://hdl.handle.net/2067/2201 2011-11-29T09:26:39Z 2010-12-31T23:00:00Z

Title: Aspetti genetico-molecolari e tecnologici del trasferimento al frumento di geni del Dasypyrum villosum Authors: Vaccino, Patrizia; Coppa, Tania; Corbellini, Maria; De Pace, Ciro Abstract: Many breeding programs rely on gene transfer from the wild and cultivated secondary gene-pool (GP-2) for trait enhancement, but so far no reports on transfer of bread-making quality-related genes from the wild GP-2 of wheat have been released. In this paper, we used small- and large-scale tests to analyze wheat introgression lines (ILs) derived from Triticum aestivum cv “Chinese Spring” (CS) Dasypyrum villosum (L.) Candargy (Dv) hybridization. We aimed at ascertaining the efficacy of prolamin subunits encoded by Dv genes for the improvement of wheat end-use grain quality Biochemical and genetic evidences from backcrossing those lines to CS, and the qualitative results obtained from the lines grown for two years at two localities demonstrated that the ascertained positive effects of the Dv genes on wheat-flour end-use quality correlate weakly with the size of the introgressed Dv chromatin that contained those genes or the protein content of the flour. Dv chromatin including genes at the Glu-V1 locus, coding for high molecular weight glutenins in Dv, significantly improves wheat bread-making quality, and chromatin from the short arm of 6V chromosome improves also protein and micronutrient (Fe and Zn) content.

2010-12-31T23:00:00Z Pasquini, Marina Bizzarri, Marco Nocente, Francesca Matere, Antonio Sereni, Loriana Casini, Fausto L'Aurora, Alessia Vaccino, Patrizia Corbellini, Maria Coppa, Tania Vittori, Doriano De Pace, Ciro http://hdl.handle.net/2067/2199 2011-10-13T23:05:32Z 2010-12-31T23:00:00Z

Title: Dasypyrum villosum come fonte di geni di resistenza a oidio e ruggine bruna per il miglioramento dei frumenti coltivati Authors: Pasquini, Marina; Bizzarri, Marco; Nocente, Francesca; Matere, Antonio; Sereni, Loriana; Casini, Fausto; L'Aurora, Alessia; Vaccino, Patrizia; Corbellini, Maria; Coppa, Tania; Vittori, Doriano; De Pace, Ciro Abstract: The wild species of cereals comprised in the secondary gene pool (GP 2)of wheat have received specific attention mainly with a view to widening the narrow genetic basis of cultivated wheats. Dasypyrum villosum Candargy (syn. Haynaldia villosa) (Dv) is an annual, diploid (2n=14), allogamous grass species, belonging to the GP 2 of wheat. This species, originating from Southern Europe and South-Western Asia, is widespread in the Mediterranean region and has been reported as carrying genes for enhancing agronomic and grain quality traits. A study has been carried out on two wheat aneuploid lines (CS V32 and CS V63) obtained after introgression of chromosome 6V # 4 into the T. aestivum cv. Chinese Spring (CS) nuclear genome by interspecific hybridization with a D. villosum population collected in Latium. The disomic addition line CS+6V carrying chromosome 6V#1 and bred by the late E.R. Sears, was also included in the study. The two aneuploid lines resulted completely resistant to Blumeria graminis f. sp. tritici (Bgt) at adult and seedling plant stage. The CS V63 line was crossed to the susceptible disomic addition line 6V # 1 and a suitable mapping population, segregating for powdery mildew resistance, was produced. The F2:3 progenies were studied both by phytopathological (with selected pathotypes of Bgt) and molecular (PCR marker) analyses in order to assess the genetic basis of resistance. The observed resistant: susceptible phenotypic ratio fitted the 3:1 ratio expected for the segregation of the resistance/susceptible alleles at one locus named “PmVt”, presumably located in 6VS. The resistant phenotype was dominant. Molecular analyses using the RAPD marker OPH171900 were carried out to confirm the location of this gene on 6VS and to verify its relationship with the Pm21 locus and association to the gliadin-encoding locus Gli-V2. The line CS V63 was tested also for resistance to Puccinia triticina (Pt): it resulted susceptible in the experiments carried out at the seedling stage with different selected Pt pathotypes. When tested with the same pathotypes in controlled infection at the adult plant stage, and in the field, in multilocation epidemiological trials, the line showed a complete or moderate resistance to this disease, thus indicating the possible presence in 6V # 4 of gene(s) for adult plant resistance to Pt.

2010-12-31T23:00:00Z Caceres, Maria Eugenia Vaccino, Patrizia Ceccarelli, Marilena Sarri, Vania Polizzi, Enza De Pace, Ciro Cionini, Pier Giorgio http://hdl.handle.net/2067/2203 2011-11-29T09:32:09Z 2010-12-31T23:00:00Z

Title: Introgressione criptica di DNA parentale in linee di frumento derivate da ibridazione intergenerica fra Triticum aestivum e Dasypyrum villosum Authors: Caceres, Maria Eugenia; Vaccino, Patrizia; Ceccarelli, Marilena; Sarri, Vania; Polizzi, Enza; De Pace, Ciro; Cionini, Pier Giorgio Abstract: Cytogenetic and DNA molecular analyses have been carried out in three wheat inbred lines (CS V58, CS V59 and CS V60), derived from Triticum aestivum cv. ‘Chinese Spring’ (CS) Dasypyrum villosum (Dv) intergeneric hybridization, which showed several Dv-specific phenotypic characters. All lines were found to share with CS the number (2n=42) and structure of the chromosomes and chromatin from Dv was apparently not added to their complement. However, Feulgen/DNA cytophotometry showed that there was more nuclear DNA in the lines than in the parental wheat (by 1.85%, 2.76% and 1.26% in CS V58, CVS V59 and CS V60, respectively). AFLP analysis of genomic DNA from the lines, CS and Dv detected a total of 120 polymorphic bands, 7 of which (5.8%) were present in both the lines and Dv but absent in CS. PCR amplification using specific primers revealed, in the genome of the lines, Dv alleles and not CS alleles at two loci (Vrn-A1 and Vrn-B3). These results indicate cryptic introgression of Dv DNA sequences into the genome of the lines. Some implications of this finding are briefly discussed.

2010-12-31T23:00:00Z Caceres, Maria Eugenia Ceccarelli, Marilena De Pace, Ciro Cionini, Pier Giorgio http://hdl.handle.net/2067/2202 2011-10-13T23:05:32Z 2010-12-31T23:00:00Z

Title: Citogenetica di ibridi fra Triticum aestivum e Dasypyrum villosum e di linee da essi derivate Authors: Caceres, Maria Eugenia; Ceccarelli, Marilena; De Pace, Ciro; Cionini, Pier Giorgio Abstract: In the course of a research aimed at the genetic improvement of wheat through intergeneric hybridization, 24 inbred breeding lines obtained by the hybridization of Triticum aestivum cv. ‘Chinese Spring’ (CS) or T. turgidum ssp. durum cv. ‘Modoc’ followed by backcross to CS have been characterized cytogenetically. Some lines were found to have the same karyotype as CS. In other lines, karyological events such as chromosome addictions, chromosome substitutions, recombinations between parental genomes or between the homoeologous genomes of wheat were observed. These findings, which were in part unexpected and even surprising, show that a careful cytogenetic characterization of wheat hybrids is necessary to understand factors and mechanisms that play a role in determining the structure of their karyotype, and then to choose strategies for the genetic improvement of wheat by interspecific and/or intergeneric hybridization.

2010-12-31T23:00:00Z