Unitus DSpace The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-24T18:15:38Z 2013-05-24T18:15:38Z The use of filamentous bacteriophage fd to deliver MAGE-A10 or MAGE-A3 HLA-A2-restricted peptides and to induce strong antitumor CTL responses Sartorius, Rossella Pisu, Paola D'Apice, Luciana Pizzella, Luciano Romano, Chiara Cortese, Giancarlo Giorgini, Angela Santoni, Angela De Berardinis, Piergiuseppe Velotti, Francesca http://hdl.handle.net/2067/1577 2011-09-26T23:05:34Z 2007-12-31T23:00:00Z Title: The use of filamentous bacteriophage fd to deliver MAGE-A10 or MAGE-A3 HLA-A2-restricted peptides and to induce strong antitumor CTL responses Authors: Sartorius, Rossella; Pisu, Paola; D'Apice, Luciana; Pizzella, Luciano; Romano, Chiara; Cortese, Giancarlo; Giorgini, Angela; Santoni, Angela; De Berardinis, Piergiuseppe; Velotti, Francesca Abstract: Delivery of tumor-associated antigen (TAA)-derived peptides in a high immunogenic form represents one of the key issues for effective peptide-based cancer vaccine development. Here, we report the ability of non-pathogenic filamentous bacteriophage fd virions to deliver HLA-A2-restricted MAGE-A10254-262- or MAGE-A3271-279-derived peptides and elicit potent specific CTL responses in vitro and in vivo. Interestingly, human anti-MAGE-A3271-279-specific CTLs were able to kill human MAGE-A3+ tumor cells, even if these cells naturally express a low amount of MAGE-A3271-279 peptide-HLA epitope surface complexes and are usually not recognized by CTLs generated by conventional stimulation procedures. MAGE-A3271-279-specific/CD8+ CTL clones were isolated from in vitro cultures and their high avidity for antigen recognition was assessed. Moreover, in vivo tumor protection assay showed that vaccination of humanized HHD (HLA-A2.1+/H2-Db+) transgenic mice with phage particles expressing MAGE-A3271-279-derived peptides hampered tumor growth. Overall, these data indicate that engineered filamentous bacteriophage virions increase substantially the immunogenicity of delivered TAA-derived peptides, thus representing a novel powerful system for the development of effective peptide-based cancer vaccines. Description: L'articolo รจ disponibile sul sito dell'editore http://www.jimmunol.org 2007-12-31T23:00:00Z