The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-19T21:06:16Z 2013-05-19T21:06:16Z Bernabucci, Umberto Colavecchia, Luciana Danieli, Pier Paolo Basiricò, Loredana Lacetera, Nicola Nardone, Alessandro Ronchi, Bruno http://hdl.handle.net/2067/1332 2011-03-18T01:30:06Z 2010-12-31T23:00:00Z

Title: Aflatoxin B1 and fumonisin B1 affect the oxidative status of bovine peripheral blood mononuclear cells Authors: Bernabucci, Umberto; Colavecchia, Luciana; Danieli, Pier Paolo; Basiricò, Loredana; Lacetera, Nicola; Nardone, Alessandro; Ronchi, Bruno Abstract: This in vitro study was focused on the effect of two widely occurring mycotoxins, aflatoxin B1 (AFB1) and fumonisin B1 (FB1), on the oxidative status of bovine peripheral blood mononuclear cells (PBMC) incubated for 2 and 7 days at different levels of AFB1 (0, 5 and 20 lg/ml) and FB1 (0, 35 and 70 lg/ml). Results of the present study demonstrate that, even though by different mechanisms, AFB1 and FB1 may induce cytotoxicity through an impairment of the oxidative status of PBMC. Lo studio in vitro ha lo scopo di verificare l’effetto della esposizione ad aflatossina B1 (AFB1) e fumonisina B1 (FB1) di sullo stato ossidativo di linfociti bovini incubati per 2 o 7 giorni con differenti concentrazioni di tossine (AFB1: 0, 5 and 20 mcg/ml and FB1: 0, 35 and 70 mcg/ml). I risultati del presente studio hanno dimostrato, anche se attraverso meccanismi differenti, che entrambe le tossine inducono citotossicità attraverso il peggioramento dello stato ossidativo.

2010-12-31T23:00:00Z Bernabucci, Umberto Colavecchia, Luciana Danieli, Pier Paolo Basiricò, Loredana Lacetera, Nicola Nardone, Alessandro Ronchi, Bruno http://hdl.handle.net/2067/1801 2011-04-01T00:30:09Z 2010-12-31T23:00:00Z

Title: Aflatoxin B1 and fumonisin B1 affect the oxidative status of bovine peripheralblood mononuclear cells Authors: Bernabucci, Umberto; Colavecchia, Luciana; Danieli, Pier Paolo; Basiricò, Loredana; Lacetera, Nicola; Nardone, Alessandro; Ronchi, Bruno Abstract: Mycotoxins are secondary metabolites having a high cytotoxic potential. They are produced by molds and released in food and feed. To date, the mechanisms underlying the mycotoxin-induced cytotoxicity have not been fully clarified. The induction of oxidative stress, as a possible mechanism, has been postulated. This in vitro study was focused on the effect of two widely occurring mycotoxins, aflatoxin B1 (AFB1) and fumonisin B1 (FB1), on the oxidative status of bovine peripheral blood mononuclear cells (PBMC) incubated for 2 and 7 days at different levels of AFB1 (0, 5 and 20 lg/ml) and FB1 (0, 35 and 70 lg/ml). Reactive oxygen metabolites (ROM), intracellular thiols (SH), malondialdehyde (MDA) and gene expression of cytoplasmic superoxide dismutase (SOD) and glutathione peroxidase (GSHPX-1) were measured on PBMC after incubation. The highest concentration of AFB1 and all concentrations of FB1 caused an increase (p < 0.05) of intracellular ROM without any time dependent effect. Intracellular SH decreased with 20 lgAFB1/ml (p < 0.05) and the effect was particularly marked after 7 days of exposure. Intracellular SH were not affected by FB1 even though a lower (p < 0.05) SH level after 2 days exposure than after 7 days was observed. MDA increased (p < 0.05) in AFB1 or FB1 treated PBMC. The exposure to FB1 for 7 days increased MDA (p < 0.05) only in cells treated with 70 lg/ml. Exposure of PBMC to AFB1 reduced SOD mRNA while FB1 decreased both SOD and GSHPX-1 mRNA abundance. These results demonstrate that, even though by different mechanisms, AFB1 and FB1 may induce cytotoxicity through an impairment of the oxidative status of PBMC.

2010-12-31T23:00:00Z