The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-21T15:05:40Z 2013-05-21T15:05:40Z Gennaro, Andrea Koebner, Robert M.D. Ceoloni, Carla http://hdl.handle.net/2067/1835 2011-06-28T11:24:31Z 2008-12-31T23:00:00Z

Title: A candidate for Lr19, an exotic gene conditioning leaf rust resistance in wheat Authors: Gennaro, Andrea; Koebner, Robert M.D.; Ceoloni, Carla Abstract: Lr19, one of the few widely effective genes conferring resistance to leaf-rust in wheat, was transferred from the wild relative Thinopyrum ponticum to durum wheat. Since Lr19 confers a hypersensitive response to the pathogen, it was considered likely that the gene would be a member of the major nucleotide-binding site (NBS)-leucine-rich repeat (LRR) plant R gene family. NBS profiling, based on PCR amplification of conserved NBS motifs, was applied to durum wheat-Thinopyrum ponticum recombinant lines involving different segments of the alien 7AgL chromosome arm, carrying or lacking Lr19. One sequence (AG15), tightly linked to Lr19, a 4,121 bp full-length cDNA was obtained. Quantitative PCR on leaf-rust infected and non-infected Lr19 carriers proved AG15 to be constitutively expressed, as is common for R genes.; L’Lr19, uno dei geni più efficaci nel conferire resistenza alla ruggine bruna del frumento, è stato trasferito dalla specie selvatica Thinopyrum ponticum al frumento duro. In considerazione del fatto che il gene Lr19 determina nell’ostite una risposta di tipo ipersensibile, è probabile che esso appartenga alla principale famiglia di geni di resistenza (R), ovvero gli NBS-LRR. L’NBS-profiling, una tecnica basata sull’amplificazione PCR dei motivi NBS più conservati, è stata applicata su linee ricombinanti frumento duro-Thinopyrum ponticum portatrici e non del gene Lr19. E’ stata isolata una sequenza completa (AG15) di cDNA lunga 4.121 bp, strettamente associata all’Lr19. Eseguendo una PCR quntitativa su linee portatrici dell’Lr19, sottoposte e non, ad infezione con ruggine bruna, si è evidenziato come la sequenza AG15 sia costitutivamente espressa, come atteso per i geni di tipo R. Description: L'articolo è disponibile sul sito dell'editore: http://www.springerlink.com.

2008-12-31T23:00:00Z Gennaro, Andrea Forte, Paola Carozza, Roberta Savo Sardaro, Luisa Maria Ferri, Daniela Bitti, Alessandra Borrelli, Grazia Maria D'Egidio, Maria Grazia Ceoloni, Carla http://hdl.handle.net/2067/1836 2011-06-30T15:04:48Z 2006-12-31T23:00:00Z

Title: Pyramiding different alien chromosome segments in durum wheat: feasibility and breeding potential Authors: Gennaro, Andrea; Forte, Paola; Carozza, Roberta; Savo Sardaro, Luisa Maria; Ferri, Daniela; Bitti, Alessandra; Borrelli, Grazia Maria; D'Egidio, Maria Grazia; Ceoloni, Carla Abstract: Wheat chromosome engineering, i.e. the transfer of alien chromosome segments from various Triticeae species into cultivated wheats, is greatly benefiting from the recent advancements in molecular genetics, cytogenetics and genomics. Powerful tools are currently available that make selection of desired genotypes far more precise and effective than in the past, thus giving this transfer strategy considerable potential for meaningful practical achievements. The paper describes the pyramiding of various disease resistance and quality-related genes, originating from different alien species, into durum wheat. Description: L'articolo é disponibile sul sito dell'editore: http://www.sciencefromisrael.com

2006-12-31T23:00:00Z Grossi, Rosaria Gennaro, Andrea Forte, Paola Bitti, Alessandra Ceoloni, Carla http://hdl.handle.net/2067/1837 2011-06-30T15:47:30Z 2008-12-31T23:00:00Z

Title: Segregation distortion following introgression of Thinopyrum ponticum into durum wheat. Authors: Grossi, Rosaria; Gennaro, Andrea; Forte, Paola; Bitti, Alessandra; Ceoloni, Carla Abstract: Segregation distortion (SD) has been observed in a wide variety of organisms, including fungi, plants, insects, and mammals. In plants, genomic regions harboring markers with abnormal segregation ratios have been reported in many crop species, including barley, pearl millet, tomato, rice, maize and wheat. Chromosomes carrying Sd genes have been also identified in several wild wheat relatives and their effect revealed upon hybridization with wheat. The case-study of this research concerns one or more Sd genes present on the long arm of 7Ag (7AgL) chromosome of the wild wheatgrass species Thinopyrum ponticum, which, once transferred in association with desirable genes into wheat chromosomes, negatively affect their segregation and also alter some phenotypic plant traits.

2008-12-31T23:00:00Z Kuzmanović, Ljiljana Gennaro, Andrea Bitti, Alessandra Ceoloni, Carla http://hdl.handle.net/2067/1851 2011-07-22T09:45:16Z 2008-12-31T23:00:00Z

Title: Pyramiding into durum and bread wheat fusarium head blight resistance and other useful traits from Thinopyrum ponticum. Authors: Kuzmanović, Ljiljana; Gennaro, Andrea; Bitti, Alessandra; Ceoloni, Carla Abstract: In recent years, Fusarium Head Blight (FHB) has become a serious problem in wheat cultivation even in areas where it was not previously present, Italy included. The severity of disease impact on grain quality and yield, and the lack of resistant cultivars within adapted germplasm, stimulated worldwide breeding efforts to develop FHB resistant wheats. In order to exploit the available genetic variability of wheat relatives, we used the wild species Thinopyrum ponticum as a source of FHB resistance to be introduced into bread and durum wheats. The ultimate goal is to pyramid the FHB resistance gene(s) with other useful genes already transferred from a different accession of the same species (Lr19+Yp, leaf rust resistance and yellow endosperm pigmentation, respectively). To this aim, suitable polymorphic DNA markers have been identified in the region of interest which facilitate the follow-up of desired alleles in the course of the multi-targeted transfer process.

2008-12-31T23:00:00Z Gennaro, Andrea Kuzmanović, Ljiljana Forte, Paola Lattanzi, Gionata Grossi, Rosaria Bitti, Alessandra Giorgi, Debora Ceoloni, Carla http://hdl.handle.net/2067/1778 2011-07-22T08:56:37Z 2009-12-31T23:00:00Z

Title: Genetic and cytogenetic maps of durum wheat-Thinopyrum ponticum recombinant chromosomes: a tool to finely describe Triticeae 7L arms and to target alien traits. Authors: Gennaro, Andrea; Kuzmanović, Ljiljana; Forte, Paola; Lattanzi, Gionata; Grossi, Rosaria; Bitti, Alessandra; Giorgi, Debora; Ceoloni, Carla Abstract: As one important result of wheat chromosome engineering, creation of arrays of wheat-alien recombinant chromosomes allows assignment of markers and genes to defined physical locations, hence improving knowledge of structural-functional organization of wheat and alien chromosomal regions. In this research we have exploited several durum wheat-Thinopyrum ponticum recombinant lines carrying relevant genes for wheat improvement.

2009-12-31T23:00:00Z Gennaro, Andrea Ceoloni, Carla http://hdl.handle.net/2067/1852 2011-06-10T10:11:16Z 2006-12-31T23:00:00Z

Title: Identification of candidate genes for traits of relevant breeding value transferred from a wild relative to wheat. Authors: Gennaro, Andrea; Ceoloni, Carla Abstract: Two valuable genes for durum wheat breeding, i.e. Lr19 (leaf-rust resistance) and Yp (yellow endosperm pigmentation), closely linked on the 7AgL chromosome arm of the perennial wheatgrass species Thinopyrum ponticum, have been transferred to the 7AL durum wheat arm by chromosome engineering. Using a ‘NBS profiling’ assay to target Lr19, and a comparative genomics approach for Yp, candidate genes for both traits have been identified.

2006-12-31T23:00:00Z Gennaro, Andrea Kuzmanović, Ljiljana Giorgi, Debora Grossi, Rosaria Bitti, Alessandra Quarrie, Steve A. Ceoloni, Carla http://hdl.handle.net/2067/1854 2011-06-29T15:06:42Z 2009-12-31T23:00:00Z

Title: Durum wheat-Thinopyrum ponticum recombinant lines: a tool to finely describe homoeologous 7L arm regions. Authors: Gennaro, Andrea; Kuzmanović, Ljiljana; Giorgi, Debora; Grossi, Rosaria; Bitti, Alessandra; Quarrie, Steve A.; Ceoloni, Carla Abstract: As one important result of wheat chromosome engineering, creation of arrays of wheat-alien recombinant chromosomes allows assignment of markers and genes to defined physical locations, hence improving knowledge of structural-functional organization of wheat and alien chromosomal regions and to identify suitable markers for MAS of target genes. Regions involved in our research are the distal portions of wheat 7AL and Thinopyrum ponticum (tall wheatgrass, a wild wheat relative) 7AgL arms, the latter containing several genes of value for wheat improvement.

2009-12-31T23:00:00Z Gennaro, Andrea Forte, Paola Lattanzi, Gionata Ferri, Daniela Carozza, Roberta D'Egidio, Maria Grazia Lafiandra, Domenico Ceoloni, Carla http://hdl.handle.net/2067/1855 2011-07-20T13:16:37Z 2008-12-31T23:00:00Z

Title: Cytogenetic and molecular characterization of durum wheat chromosome transfers with 1D-associated gluten protein genes and their pyramiding Authors: Gennaro, Andrea; Forte, Paola; Lattanzi, Gionata; Ferri, Daniela; Carozza, Roberta; D'Egidio, Maria Grazia; Lafiandra, Domenico; Ceoloni, Carla Abstract: Gluten quality of bread wheat is known to be mainly associated with high- (HMW-GS) and low-(LMW-GS) molecular weight glutenin subunits encoded by Glu-1 (L arm of group-1 chromosomes) and Glu-3 (S arm of group-1 chromosomes) genes, respectively, with the 1D alleles of such genes having the major impact on bread making properties. Transfer of chromosomal segments containing the Glu-D1 and Gli-D1/Glu-D3 loci was successfully achieved in a number of instances resorting to chromosome engineering. Using this strategy, we isolated two 1A-1D recombinant lines, in which the Gli-D1/Glu-D3 genes and the Glu-D1d allele (HMW-GS "5+10") were separately transferred into the 1AS and 1AL arm, respectively, of recipient durum wheat lines (named PS and PL, respectively). Also, a detailed genetic map of both recombinant chromosome arms was developed. Stable PS + PL double-recombinant lines have been obtained as a result of homologous recombination in the 1A portions shared by the two recombinant chromosomes present in PS x PL hybrids. Preliminary quality tests suggest that the Glu-D3 + Glu-D1d combined presence could determine a slight increase of gluten quality parameters over those associated with Glu-D1d alone.

2008-12-31T23:00:00Z