The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-23T02:25:22Z 2013-05-23T02:25:22Z Salinas, Irene Abelli, Luigi Bertoni, Fabrizio Picchietti, Simona Roque, Ana Furones, Dolors Cuesta, Alberto Meseguer, José Esteban, Maria Ángeles http://hdl.handle.net/2067/1649 2011-07-04T19:47:55Z 2007-12-31T23:00:00Z

Title: Monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead seabream (Sparus aurata L.) Authors: Salinas, Irene; Abelli, Luigi; Bertoni, Fabrizio; Picchietti, Simona; Roque, Ana; Furones, Dolors; Cuesta, Alberto; Meseguer, José; Esteban, Maria Ángeles Abstract: The effects of the oral administration of heat-inactivated Lactobacillus delbru¨eckii ssp. lactis and Bacillus subtilis, individually or combined, on gilthead seabream immune responses were investigated both systemically and locally in the gut. In a first experiment, seabream (65 g) were fed for 3 weeks different diets supplemented with 1 107 CFU g 1 Lactobacillus, 1 107 CFU g 1 Bacillus, or 0.5 107 CFU g 1 Lactobacillus plus 0.5 107 CFU g 1 Bacillus. Controls were fed non-supplemented diet. Six fish per group were sampled at the end of the trial and some humoral and cellular systemic innate immune parameters were evaluated. Feeding the mixture of the two killed bacteria species significantly increased natural complement, serum peroxidase and phagocytic activities compared with controls. In a second experiment, juvenile seabream (13 g) were fed for 3 weeks the same experimental diets and total serum IgM and numbers of gut IgMþ cells and acidophilic granulocytes were evaluated. All these parameters were significantly higher in the multispecies probiotic group compared to monospecies and control fed groups. Description: L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com

2007-12-31T23:00:00Z Picchietti, Simona Guerra, Laura Bertoni, Fabrizio Randelli, Elisa Belardinelli, Maria Cristina Buonocore, Francesco Rombout, Jan Scapigliati, Giuseppe Abelli, Luigi http://hdl.handle.net/2067/2183 2011-08-04T23:05:11Z 2010-12-31T23:00:00Z

Title: Intestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studies Authors: Picchietti, Simona; Guerra, Laura; Bertoni, Fabrizio; Randelli, Elisa; Belardinelli, Maria Cristina; Buonocore, Francesco; Rombout, Jan; Scapigliati, Giuseppe; Abelli, Luigi Abstract: Cellular and molecular data have evidenced a gut-associated lymphoid tissue in a variety of teleost species, abundantly containing T cells, whose origin, selection and functions are still unclear. This study reports CD4, CD8-α, MHCI-α, MHCII-β, rag-1 and TCR-β gene transcription along the intestine (anterior, middle and posterior segments) and in the thymus of one year-old Dicentrarchus labrax (L.). Real-time PCR findings depicted a main role of the thymus in T cell development, but also rag-1 and CD8-α transcripts are detected in the intestine, having significant expression in the posterior segment. In the whole intestine TCR-β and CD8-α exceeded CD4 transcripts. RNA ISH confirmed these data and detailed that mucosal CD8-α+ cells were especially numerous in the epithelium and in aggregates in the lamina propria. Regional differences in T cell-specific gene expressions are first described in the intestine of a bony fish. High non-specific cytotoxic activity against xenogeneic and allogeneic cells was found in lymphocytes purified from the intestinal mucosa, providing further insight into their local defence roles. Description: L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/

2010-12-31T23:00:00Z