The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://http://dspace.unitus.it:80 2013-05-23T16:20:14Z 2013-05-23T16:20:14Z Amore, Valentina http://hdl.handle.net/2067/1073 2012-03-23T14:29:41Z 2010-03-14T23:00:00Z

Title: Molecular approach to the study of hemocyanin in stoneflies (Plecoptera): a biologic and phylogenetic interpretation Authors: Amore, Valentina Abstract: Contrary to what was assumed regarding the presence of respiratory proteins in insects, a functional hemocyanin was recently found in larvae and adults of the stoneflies species Perla marginata in P. grandis. In order to verify if the presence of this ancient trait is widespread within the order and to investigate why stoneflies have maintained it, we have extended the search for hemocyanin within Plecoptera families. In particular, we assessed the presence of hemocyanin in the larval and adult stage the seven families of the European stonefly fauna, and we tested some taxa of Oriental and Southern African fauna living in environments with different ecological features respect to those of the Palaearctic streams. We cloned and sequenced cDNAs corresponding to the conserved hemocyanin fragment (in domain II) and studied their expression with PCR technique. To evaluate hemocyanin presence as translated product, highly sensitive method based on liquid chromatography tandem mass spectrometry, was used. Phylogenetic analyses were inferred. On the basis of our outcomes, we have revealed that hemocyanin seems to be not uniformly distributed within this order. Phylogenetic relations, environmental induction and biological aspects, such as larval size, life cycle length and trophic roles, are discussed as possible factors that may be correlated with the presence or absence of hemocyanin in the studied species. Moreover, we take into account the potential multifunctionality of hemocyanin. In this work we presented the current knowledge about the hemocyanin in the Plecoptera as outcomes of our research.; Diversamente dalle conoscenze attuali sulla presenza delle proteine respiratorie negli insetti, è stata recentemente ritrovata emocianina in larve ed adulti delle specie di plecotteri Perla marginata in P. grandis. Allo scopo di verificare se la presenza di questo carattere antico è diffuso all’interno dell’ordine e per indagare sulle motivazione per cui i plecotteri lo hanno mantenuto, abbiamo esteso la ricerca dell’emocianina all’interno di questo gruppo. In special modo, abbiamo comprovato la presenza in larve ed adulti nelle sette famiglie della fauna europea, ed inoltre abbiamo saggiato qualche taxa della fauna orientale e sudafricana, caratterizzate da condizioni ecologiche differenti rispetto a quelle dei fiumi paleartici. Abbiamo clonato e sequenziato cDNA corrispondenti al frammento conservato di emocianina (nel dminio II), ed abbiamo studiato la loro espressione con tecniche di PCR. Per accertare la presenza di emocianina come prodotto di traduzione, si è utilizzato un metodo altamente sensibile basato sulla spettrometria di massa. Infine la presenza dell’emocianina è stata analizzata in chiave filogenetica. Sulla base dei risultati ottenuti, abbiamo messo evidenza che l’emocianina sembra non essere uniformemente distribuita all’interno dell’ordine. Le relazioni filogenetiche, l’induzione ambientale ed alcuni caratteri biologici, come la grandezza delle larve, la durata del ciclo biologico ed il ruolo trofico, sono state discusse come possibili fattori che possono essere correlati con la presenza o l’assenza dell’emocianina nelle specie studiate. Inoltre sono state considerati le possibili multifunzioni dell’emocianina. Nel presente lavoro viene raccolto lo stato di attuale conoscenza sul tema dell’emocianina nei plecotteri, frutto di questa ricerca. Description: Dottorato di ricerca in Ecologia e gestione delle risorse biologiche

2010-03-14T23:00:00Z Amore, Valentina Gaetani, Brunella Puig, Maria Ángels Fochetti, Romolo http://hdl.handle.net/2067/1452 2011-06-28T14:31:16Z 2010-12-31T23:00:00Z

Title: New data on the presence of hemocyanin in Plecoptera: recomposing a puzzle Authors: Amore, Valentina; Gaetani, Brunella; Puig, Maria Ángels; Fochetti, Romolo

2010-12-31T23:00:00Z Amore, Valentina Belardinelli, Maria Cristina Guerra, Laura Buonocore, Francesco Fausto, Anna Maria Fochetti, Romolo http://hdl.handle.net/2067/1426 2011-06-13T10:44:47Z 2008-12-31T23:00:00Z

Title: Do all stoneflies nymphs have respiratory proteins? Further data on the presence of hemocyanin in the larval stages of plecoptera species Authors: Amore, Valentina; Belardinelli, Maria Cristina; Guerra, Laura; Buonocore, Francesco; Fausto, Anna Maria; Fochetti, Romolo Description: L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com

2008-12-31T23:00:00Z Fochetti, Romolo Amore, Valentina Gaetani, Brunella http://hdl.handle.net/2067/1417 2011-08-08T23:05:22Z 2010-12-31T23:00:00Z

Title: Lack of Hemocyanin in Oriental Plecoptera and Multifuncionality of the Protein in Larvae Authors: Fochetti, Romolo; Amore, Valentina; Gaetani, Brunella

2010-12-31T23:00:00Z Fochetti, Romolo Amore, Valentina Ubero-Pascal, Nicolás Egidi, Maria Giulia Timperio, Anna Maria http://hdl.handle.net/2067/1415 2011-06-28T10:18:45Z 2010-12-31T23:00:00Z

Title: Comparative Proteomic Analysis of Hemocyanins in Dinocras cephalotes and Perla marginata (Plecoptera) Authors: Fochetti, Romolo; Amore, Valentina; Ubero-Pascal, Nicolás; Egidi, Maria Giulia; Timperio, Anna Maria Abstract: Hemocyanins are large oligomeric respiratory proteins found in many arthropods and mollusks. The overall expression of hemocyanin mRNA, revealed by studies on Plecoptera hemocyanin sequencing, has raised the question of whether the protein is expressed or not. In fact, the presence of expressed hemocyanin has only been reported in the literature for one species, Perla marginata (Panzer, 1799). In this paper, we report the presence of hemocyanin and hexamerin proteins in Dinocras cephalotes (Curtis, 1827), a species closely related to P. marginata. To assess the presence of hemocyanin, we used a reproducible and highly sensitive method based on liquid chromatography tandem mass spectrometry. We conclude that regardless of its putative function (respiratory, immune defense, storage protein), the hemocyanin is actually expressed in species in which its mRNA is present. Description: L'articolo è disponibile sul sito dell'editore http://www.entsoc.org

2010-12-31T23:00:00Z Puig, Maria Ángels Amore, Valentina Ubero-Pascal, Nicolás Fochetti, Romolo http://hdl.handle.net/2067/1445 2011-06-16T09:56:23Z 2009-12-31T23:00:00Z

Title: Biodiversitat de plecòpters i efemeròpters al Parc Nacional d'Aigüestortes i Estany de Sant Maurici Authors: Puig, Maria Ángels; Amore, Valentina; Ubero-Pascal, Nicolás; Fochetti, Romolo

2009-12-31T23:00:00Z Fochetti, Romolo Amore, Valentina http://hdl.handle.net/2067/1418 2011-07-04T19:50:32Z 2008-12-31T23:00:00Z

Title: Present knowledge on the presence of hemocyanin in stoneflies (Insecta: Plecoptera) Authors: Fochetti, Romolo; Amore, Valentina Abstract: Hemocyanin is a respiratory protein that occurs in the main lineages of Arthropoda. In insects hemocyanin is presently known in many orders. Recently, a functional hemocyanin has also been found in the Plecoptera. Further studies have revealed that hemocyanin seems to be not uniformly distributed within this order. In this paper we report additional data, obtained with RT-PCR sequencing, on the presence of hemocyanin in different stonefly species. In addition, we summarise the present knowledge about the distribution of hemocyanin in the Plecoptera. Biological aspects such as larval size, life cycle length, trophic roles and environmental induction are discussed as possible factors that may be correlated with the presence or absence of hemocyanin in the studied species. Description: L'articolo è disponibile sul sito dell'editore http://www.tandf.co.uk/journals/default.asp

2008-12-31T23:00:00Z