DSpace Community:http://hdl.handle.net/2067/13042024-02-14T23:20:53Z2024-02-14T23:20:53ZTranscription of T cell-related genes in teleost fish, and the European sea bass (Dicentrarchus labrax) as a modelBoschi, IleniaRandelli, ElisaBuonocore, FrancescoCasani, DanielaBernini, ChiaraFausto, Anna MariaScapigliati, Giuseppehttp://hdl.handle.net/2067/22052011-10-13T23:05:33Z2011-01-01T00:00:00ZTitle: Transcription of T cell-related genes in teleost fish, and the European sea bass (Dicentrarchus labrax) as a model
Authors: Boschi, Ilenia; Randelli, Elisa; Buonocore, Francesco; Casani, Daniela; Bernini, Chiara; Fausto, Anna Maria; Scapigliati, Giuseppe
Abstract: In recent years the cloning of genes coding for immuno-regulatory peptides, as well as the sequencing of genomes, provided fish immunologists with a growing amount of information on nucleotide sequences. Research is now also addressed in investigating the functional immunology counterpart of nucleotide sequence transcripts in various fish species. In this respect, studies on functional immunology of T cell activities are still at their beginning, and much work is needed to investigate T cell responses in teleost fish species.
In this review we summarise the current knowledge on the group of genes coding for main T cell-related peptides in fish, and the expression levels of these genes in organs and tissues. Particular attention is paid to European sea bass (Dicentrarchus labrax), a marine species in which some information on functional immunology has been obtained, and we reassume here the expression of some T cell-related genes in basal conditions. In addition, we provide original data showing that T cells purified from the intestinal mucosa of sea bass with a specific mAb, express transcripts for TRgamma, TRbeta, CDalpha and RAG-1, thus showing similarities with intraepithelial leucocytes of mammals.
Description: L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com2011-01-01T00:00:00ZCD3 γ/δ in sea bass (Dicentrarchus labrax): molecular characterization and expression analysisRandelli, ElisaScapigliati, GiuseppeBuonocore, Francescohttp://hdl.handle.net/2067/22042020-09-17T00:05:19Z2011-01-01T00:00:00ZTitle: CD3 γ/δ in sea bass (Dicentrarchus labrax): molecular characterization and expression analysis
Authors: Randelli, Elisa; Scapigliati, Giuseppe; Buonocore, Francesco
Abstract: The CD3 complex is the common marker on the surface of both ab and gd T cells and is essential for
formation of the T-cell receptor complex and for T-cell activation.
In this paper, we report the gene cloning and molecular characterization of a CD3g/d homologue in
sea bass (Dicentrarchus labrax), the analysis of transcription levels in lymphoid and non-lymphoid
organs and the gene regulation after in vitro stimulation with LPS and PHA.
Four cysteine residues in the extracellular domain, involved in the constitution of immunoglobulinlike
domain, are present in sea bass CD3g/d sequence and they are conserved both in number and
position from mammals to teleost sequences. Similar to other known CD3g/ds, in sea bass CD3g/d there
is also a conserved immunoreceptor tyrosine-based activation ITAM motif that could be responsible for
its individual signal transduction capacity.
The real time RT-PCR basal analysis shows the highest level of CD3g/d mRNA in thymus, followed by
peripheral blood leucocytes, spleen, gills, gut, liver, head kidney, brain and muscle. The expression
analysis under stimuli condition reveals a significant decrease of CD3g/d expression after LPS
stimulation and a significant increase after PHA-L stimulation, in agreement with mammals results.
In conclusion, these data allow us to affirm that sea bass CD3g/d can be used as a T cell marker and
will help in adding new insight on the immune response mechanisms of sea bass.
Description: L'articolo è disponibile sul sito dell'editore http://www.elsevier.com2011-01-01T00:00:00ZIntestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studiesPicchietti, SimonaGuerra, LauraBertoni, FabrizioRandelli, ElisaBelardinelli, Maria CristinaBuonocore, FrancescoRombout, JanScapigliati, GiuseppeAbelli, Luigihttp://hdl.handle.net/2067/21832011-08-04T23:05:11Z2011-01-01T00:00:00ZTitle: Intestinal T cells of Dicentrarchus labrax (L.): gene expression and functional studies
Authors: Picchietti, Simona; Guerra, Laura; Bertoni, Fabrizio; Randelli, Elisa; Belardinelli, Maria Cristina; Buonocore, Francesco; Rombout, Jan; Scapigliati, Giuseppe; Abelli, Luigi
Abstract: Cellular and molecular data have evidenced a gut-associated lymphoid tissue in a variety of teleost species, abundantly containing T cells, whose origin, selection and functions are still unclear. This study reports CD4, CD8-α, MHCI-α, MHCII-β, rag-1 and TCR-β gene transcription along the intestine (anterior, middle and posterior segments) and in the thymus of one year-old Dicentrarchus labrax (L.).
Real-time PCR findings depicted a main role of the thymus in T cell development, but also rag-1 and CD8-α transcripts are detected in the intestine, having significant expression in the posterior segment. In the whole intestine TCR-β and CD8-α exceeded CD4 transcripts. RNA ISH confirmed these data and detailed that mucosal CD8-α+ cells were especially numerous in the epithelium and in aggregates in the lamina propria. Regional differences in T cell-specific gene expressions are first described in the intestine of a bony fish.
High non-specific cytotoxic activity against xenogeneic and allogeneic cells was found in lymphocytes purified from the intestinal mucosa, providing further insight into their local defence roles.
Description: L'articolo è disponibile sul sito dell'editore http://www.sciencedirect.com/2011-01-01T00:00:00ZAnalysis of the expression and modulation of selected immune-related gene transcripts in the DLEC cell line from European sea bass (Dicentrarchus labrax)Buonocore, FrancescoRandelli, ElisaLorenzen, NielsEiner-Jensen, KatjaScapigliati, Giuseppehttp://hdl.handle.net/2067/21802020-07-03T02:06:02Z2011-01-01T00:00:00ZTitle: Analysis of the expression and modulation of selected immune-related gene transcripts in the DLEC cell line from European sea bass (Dicentrarchus labrax)
Authors: Buonocore, Francesco; Randelli, Elisa; Lorenzen, Niels; Einer-Jensen, Katja; Scapigliati, Giuseppe
Abstract: Cell lines have been established from different fish species especially for virus isolation and for studying cell-pathogen
interactions, and therefore are of interest in aquaculture. In this paper, we have investigated the presence and the
regulation of some immune genes in the DLEC (Dicentrarchus labrax embryonic cells) cell line from European sea bass
(Dicentrarchus labrax L.) to preliminary elucidate their action. The basal expression of the selected genes (interleukin-
1β (IL-1β), cyclooxygenase-2 (COX-2), transforming growth factor-β (TGF-β), CD8-α, major histocompatibility complex
II-β (MHC II-β), interferon (IFN) and Mx protein (Mx)) have been investigated and, successively, their modulation have
been studied both after stimulation with different mitogen agents and after a transfection with a sequence codifying for
the coat protein of a fish nervous necrosis virus (NNV). The results have evidenced that the inflammatory molecules
(IL-1β, COX-2, TGF-β), constitutively expressed by the DLEC cell line, are not up-regulated by the stimulation with
lipopolysaccharide (LPS) from E. coli, whether the expression of the T-cell marker transcripts (CD8-α, MHC II-β) is
influenced by the action of a lectin from Phaseolus vulgaris (PHA-L). Finally, the expression of the coat NNV protein
in the DLEC cell line, after the transfection, led to an high up-regulation of IFN and Mx gene transcripts. These data
suggest that the DLEC cell line recognize specific pathogen-associated molecular patterns (PAMPs) and, therefore,
could be useful for studying T-cell pathways and viral responses in sea bass avoiding the use of live test animals.2011-01-01T00:00:00Z